Solid Phase Extraction (SPE) and QuEChERS
Solid Phase Extraction (SPE) is one of the most popular analytical sample preparation techniques of the current times. It is designed for preparing rapid and selective samples for subsequent chromatographic analysis. In SPE, one or more analytes from a liquid sample are isolated by extracting, partitioning, and/or adsorbing onto a solid stationary phase.
Explore our wide range of products that include individual cartridges, 96-well plates, QuEChERS tubes (dispersive SPE), and LiChrolut® extraction columns and tubes. Each one is packed with a distinct phase chemistry which can be processed using a vacuum manifold and dedicated accessories.
Section Overview
SPE Cartridges
We offer a diverse range of SPE-phase chemistries and cartridges ideal for many applications including pharmaceutical, clinical, food and beverage, environmental, petrochemical, and agriculture. The phase chemistry selection allows users to screen for the retention and selectivity required to achieve sample preparation objectives.
SPE Well Plates
Our SPE plates allow users to process up to 96 samples simultaneously for increased reproducibility and throughput, while maintaining excellent recoveries and increased sensitivity. 96-well plates offer compatibility with multi-channel pipettors and automation and are available with silica sorbent and polymeric adsorbent.
QuEChERS
The “QuEChERS” (Dispersive SPE, dSPE) method has emerged as a popular sample preparation technique in the area of multi-residue pesticide analysis for food and agricultural products. The QuEChERS technique offers the advantage of higher recoveries, accurate results, and high sample throughput.
DPX® Tips
Dispersive Pipette Extraction (DPX) tips provide an in-tip solution for complete sample preparation that can be easily automated to achieve extraction in seconds. HybridSPE® sorbent material is loosely contained within a tip, for selective retention and removal of endogenous phospholipid interferences from biological matrices for LC-MS or LC-MS/MS analysis.
LiChrolut® Extraction Columns & Tubes
LiChrolut® extraction columns and tubes use strong but reversible interactions between the analyte and the surface of the stationary. Typical interactions include hydrophobic (Van-der-Waals forces), polar (hydrogen bonding, dipole-dipole forces) or ion exchange interactions, and assure reliable and economical solid phase extractions
Related Product Resources
- Flyer: NEW – PTFE-Free Visiprep™ SPE Vacuum Manifolds, Suitable for PFAS Testing Workflow
Our updated Visiprep™ standard 12 and 24-port vacuum manifolds are suitable for sample preparation processes in the PFAS testing workflow.
- Article: Simplified LC-MS/MS Method for Glyphosate, AMPA, and Glufosinate in Oat-Based Cereals
Glyphosate analysis: LC-MS/MS method with Supel™ Carbon LC U/HPLC column for stability and retention.
- Article: Analysis of Drugs of Abuse in Urine After Cleanup with New Supel™ Swift HLB Solid Phase Extraction 96-well plates
Validate urine sample cleanup with HLB SPE for opioid analysis via LC-MS/MS using the Supel™ Swift HLB 96-well plate.
- Application Note: LC-MS Analysis of PFAS Compounds in EPA 533 after Supelclean™ ENVI-WAX™ SPE Cleanup
PFAS compounds are also commonly known as “forever chemicals” which means they do not break down in the environment like other chemicals. This persistence can result in the concentration of these compounds growing to levels that are unsafe for human exposure and negative health effects.
- Brochure: Solid Phase Extraction Products
Over the last twenty five years, SPE has become the most powerful technique available for rapid and selective sample preparation (prep) prior to analytical chromatography.
- Brochure: Supel™ QuE QuEChERS
In “QuEChERS” methodology, the use of loose extraction salts and cleanup sorbents in combination with shaking and centrifugation results in a Quick, Easy, Cheap, Effective, Rugged and Safe sample cleanup technique.
- Brochure: HybridSPE® Phospholipid Removal Technology for Biological Matrices
Phospholipids are present as a major component of all cell membranes. They are therefore present in all biological sample matrices including serum, plasma and whole blood and can be a problem in LC-MS analysis of small molecules because they often co-elute and ionize along with the analytes of interest.
- Flyer: Supel™ Swift HLB – DPX Tips
Supel™ Swift HLB SPE is a new, proprietary, and patent pending copolymer having both hydrophilic and lipophilic functional groups.
- Application Note: Simplified LC-MS/MS Method for Glyphosate, AMPA and Glyfosinate in Oat-based Cereals
A LC-MS/MS method with simple SPE interference removal for the determination of glyphosate, (aminomethyl)phosphonic acid (AMPA), and glufosinate in cereals is described.
- Selection Guide: Supel™ QuE for QuEChERS
The “QuEChERS” method has emerged as a sample prep technique popular for multi-residue pesticide analysis in food and agricultural products, and is formalized in the methods EN 15662 and AOAC 2007.01.
- Flyer: DPX HybridSPE® Tips
DPX stands for Dispersive Pipette Extraction, a patented technology that introduces the benefits of solid phase extraction into a revolutionary, easy-to-use pipette tip.
- Flyer: Supel™ Swift HLB SPE Cartridges
Supel™ Swift HLB SPE is a polymeric stationary phase for solid phase extraction prior to instrumental analysis. It has both hydrophilic and lipophilic functional groups for the extraction of a broad range of compounds from aqueous samples.
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