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  • Serum modifies the concentration-dependent effects that sex steroids exert on cgmp and cAMP levels, and the growth of human c4-I cells (carcinoma of the uterine cervix).

Serum modifies the concentration-dependent effects that sex steroids exert on cgmp and cAMP levels, and the growth of human c4-I cells (carcinoma of the uterine cervix).

International journal of oncology (1994-09-01)
A Orbo, R Jaeger, G Sager
ABSTRACT

About 50% of the cases with invasive carcinoma of the uterine cervix occurs before menopause. Cyclic changes of sex steroids may therefore interfere with the ratio between extracellular levels of cGMP and cAMP (cGMP(ex)/cAMP(ex)). This ratio has been proposed as a diagnostic marker of malignant diseases. In the present study, we tested the ability of sex steroids to affect cAMP and cGMP levels after 96 h exposure, in an established in vitro model of carcinoma of the uterine cervix (C4-I cells). Serum may modify the effects of sex steroids, and due to this, the present experiments were performed both in a chemically defined medium (CDM) and a serum-supplemented medium (SSM). Testosterone caused a concentration-dependent increase of cGMP(ex)/cAMP(ex) in SSM. Progesterone caused a concentration-dependent inhibition of cell growth in SSM and a decrease in cGMP(ex)/cAMP(ex). Progesterone had noed a concentration-dependent increase in cGMP(ex)/cAMP(ex). No distinct effects on, were seen in the other situations. The present study demonstrates that steroids affect the distribution of cGMP and cAMP to the extracellular compartment, and that serum modifies these effects.

MATERIALS
Product Number
Brand
Product Description

Supelco
ORBO 402 Tenax® TA specially treated (35/60), 100/50 mg, W,W,W separators, O.D. × L 8 mm × 100 mm, pkg of 50 ea
Supelco
ORBO 609 Amberlite XAD®-2 (20/50) 400/200 mg, W,W,W separators, O.D. × L 8 mm × 110 mm, pkg of 50 ea
Supelco
ORBO 53 Activated Silica Gel (20/40) specially cleaned, 400/200 mg with Glass Fiber Filters, GFF,F,F separators, O.D. × L 7 mm × 100 mm, pkg of 50 ea
Supelco
ORBO 554 H2SO4 on Silica Gel (20/40) 150/75 mg, W,W,W separators, O.D. × L 6 mm × 75 mm, pkg of 50 ea
Supelco
ORBO 23 2-HMP on Amberlite XAD®-2 (20/40), 120/60 mg, W,W,W separators, O.D. × L 6 mm × 85 mm, pkg of 25 ea
Supelco
ORBO 502 Activated Silica Gel (20/45) 100/50 mg, W,W,F separators, O.D. × L 6 mm × 75 mm, pkg of 50 ea
Supelco
ORBO 80 Coated Filter, with cassettes unassembled
Supelco
ORBO 90 Carboxen® 564 (20/45), 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)
Supelco
ORBO 65P (OVS) Amberlite XAD®-4 specially cleaned, 160/80 mg, RC,GFF,F,F (specially cleaned) separators, O.D. × L 13-8 mm × 75 mm , OSHA Versatile Sampler (OVS), pkg of 10 ea
Supelco
ORBO 80 Isocyanate Filter, with cassettes: no, filter O.D. 37 mm, pkg of 25 ea
Supelco
ORBO 52 Small Activated Silica Gel (20/40) 150/75 mg, pk 50, W,W,F separators, O.D. × L 6 mm × 75 mm, pkg of 50 ea
Supelco
ORBO 92 Carboxen® 564 (20/45), 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group Vinyl acetate
Supelco
ORBO 43 Supelpak-20 specially treated Amberlite XAD®-2 (20/40), 100/50 mg, W,W,W (specially treated) separators, O.D. × L 8 mm × 100 mm, pkg of 50 ea
Supelco
ORBO 24 2-HMP on Amberlite XAD®-2 (20/40), 150/75 mg, W,W,W separators, O.D. × L 6 mm × 105 mm, pkg of 25 ea