Skip to Content
Merck
  • Development of a cloud point extraction method for the determination of phenolic compounds in environmental water samples coupled with high-performance liquid chromatography.

Development of a cloud point extraction method for the determination of phenolic compounds in environmental water samples coupled with high-performance liquid chromatography.

Journal of separation science (2012-08-30)
Huihui Ma, Fengtian Mu, Shujuan Fan, Xiao Zhou, Qiong Jia
ABSTRACT

A method based on ultrasound-assisted cloud point extraction (CPE) was developed for the determination of phenolic compounds in water samples by high-performance liquid chromatography coupled with diode array detection (DAD) (HPLC-DAD). A nonionic surfactant, Tergitol 15-S-7, was chosen as the surfactant. The parameters affecting the CPE efficiency, such as Tergitol 15-S-7 concentration, salt concentration, extraction temperature, incubation time, centrifugation time, ultrasonic time, sample pH, and dilution solvent were systematically evaluated and optimized. Under the optimum experimental conditions, the linear regression coefficients of the standard curves were greater than 0.9986 and the limits of detection were in the range of 1.7-6.0 μg/L. The method was shown to be reproducible and reliable with intraday and interday relative standard deviations lower than 4.0 and 5.8%, respectively. The recoveries for the addition of different concentrations of phenolic compounds to water samples were in the range of 81.1-109.4%.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poloxamer 188, micronized
Sigma-Aldrich
Poloxamer 188 solution, 10%, sterile-filtered, BioReagent, suitable for insect cell culture
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~2,000
Sigma-Aldrich
TERGITOL, Type NP-10
Sigma-Aldrich
Poloxamer 407, micronized
Sigma-Aldrich
Poloxamer 188
Sigma-Aldrich
Poloxamer 407, oxyethylene 71.5-74.9 %
Sigma-Aldrich
Synperonic® PE/P84
Sigma-Aldrich
Poloxamer 407, purified, non-ionic
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~1,900
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~2,900
Sigma-Aldrich
Poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol), average Mn ~2,000
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~5,800
Sigma-Aldrich
Synperonic® PE P105, surfactant
Sigma-Aldrich
Synperonic® F 108, surfactant, non-ionic
Sigma-Aldrich
Poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol), average Mn ~3,300
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~14,600
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~8,400
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~1,100
Sigma-Aldrich
Pluronic® F-127, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Poloxamer 188, solid
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~4,400