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  • Universal efavirenz determination in transport study, rat placenta perfusion and placenta lysate by HPLC-UV.

Universal efavirenz determination in transport study, rat placenta perfusion and placenta lysate by HPLC-UV.

Journal of pharmaceutical and biomedical analysis (2017-01-17)
Lucie Zelena, Josef Reznicek, Martina Ceckova, Hana Sklenarova
PMID28092857
ABSTRACT

Efavirenz is an antiretroviral drug used in the treatment of HIV-positive patients. A simple, fast and sensitive high-performance liquid chromatography (HPLC) method was developed in order to determine efavirenz in three types of samples provided from pharmacokinetic studies. The analysis took 5min and was performed using a C18 analytical column (Discovery HS C18, 150×4.6mm, particle size of 5μm) in isocratic mode with a mobile phase containing acetonitrile and water (65:35, v/v), a flow rate of 1.6mLmin

MATERIALS
Product Number
Brand
Product Description
581394-U
Supelco
Discovery® HS C18 Prep Guard Cartridge, 10 μm particle size, L × I.D. 1 cm × 21.2 mm
568533-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
568520-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
568512-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4 mm
568513-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
568521-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
568543-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm
568500-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm
568502-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
568643-U
Supelco
Discovery® HS C18 HPLC Column, 10 μm particle size, L × I.D. 25 cm × 21.2 mm
568501-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
568523-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
568522-U
Supelco
Discovery® HS C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
569250-U
Supelco
Discovery® HS C18 HPLC Column, 3 μm particle size, L × I.D. 5 cm × 4.6 mm
569255-U
Supelco
Discovery® HS C18 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 2.1 mm
569254-U
Supelco
Discovery® HS C18 HPLC Column, 3 μm particle size, L × I.D. 7.5 cm × 2.1 mm
569253-U
Supelco
Discovery® HS C18 HPLC Column, 3 μm particle size, L × I.D. 5 cm × 2.1 mm
569252-U
Supelco
Discovery® HS C18 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
569251-U
Supelco
Discovery® HS C18 HPLC Column, 3 μm particle size, L × I.D. 7.5 cm × 4.6 mm