813125
TSKgel® Boronate-5PW (20 µm) HPLC Columns
L x I.D. 2.5 cm × 6 mm, m-aminophenyl boronate phase
Synonym(s):
TSKgel® Boronate-5PW HPLC Guardgel Kit
About This Item
Recommended Products
product name
TSKgel® Boronate-5PW HPLC Guardgel Kit, phase m-aminophenyl boronate, L × I.D. 2.5 cm × 6 mm, 20 μm particle size
material
PEEK
product line
TSKgel®
manufacturer/tradename
Tosoh Boronate-5PW, 13125
parameter
≤20% organic solvent
≤3 M salt concentration
≥ 0 °C temperature
technique(s)
HPLC: suitable
L × I.D.
2.5 cm × 6 mm
matrix
polymer particle platform
matrix active group
m-aminophenyl boronate phase
particle size
20 μm
pore size
1000 Å
operating pH
2-9
capacity
40 μmol/mL, resin adsorption capacity (sorbitol)
compatibility
mode of use affinity HPLC
separation technique
affinity
storage temp.
2-8°C
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General description
High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.
Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
Components
- 5 mL Guardgel packing material
- Column holder, consisting of external reducing union, cap, frit, 2 ferrules
- Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 μm frit at 10 mm depth, 1/16"
- 2 Replacement frits
- Capillary tubing, 5 cm x 1/16" OD x 0.01"
- Nuts and ferrules, 10-32 threads for 1/16" tubing, 2 each
- All hardware is 316 stainless steel
Dimensions for Guardgel kits for 21.5 mm ID columns:
- Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 μm frit at 20mm depth, 1/16"
- Capillary tubing, 5 cm x 1/16" OD x 0.02" ID
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