Isopropanol Molecular Biology Reagent

Product No. I9516

CAS RN 67-63-0
EC 200-661-7
Synonyms: 2-Propanol, sec-Propyl alcohol, IPA, Isopropyl alcohol

Product Description

Isopropanol is a polar organic solvent that is widely used in chemistry, biochemistry and molecular biology. Industrial applications include its use in antifreezes, quick-drying oils, and quick-drying inks, and as a solvent for gums, shellac, and essential oils.¹ Isopropanol has been utilized as a solvent in the synthesis of solid phase acylating agents.²

Protocols for the use of isopropanol in DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues have been reported.³ Isopropanol has been utilized in protein crystallization.^4,5,6 The use of isopropanol to isolate retinoids from biological matrices for HPLC and HPLC-MS analysis has been reported.^7,8 A mathematical analysis of isopropanol and other organic solvents to examine the selectivity of stationary phases and organic modifiers in reversephase HPLC systems has been published.⁹

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Preparation Instructions

This product is miscible in water, alcohol, ether, and chloroform.¹

Product Profile

Molecular Formula: C₃H₈O
Molecular Weight: 60.10
Boiling Point: 82.5 °C (760 torr)¹
Density: 0.78505 g/mL (20 °C)

Specificity

This product is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

References

1. Editor Susan B, Maryadele J. O, Patricia E. H, Ann S, J. Budavari O, Joanne F. K, Budavari S. 1996. The Merck Index. Twelfth Edition, Entry# 5227..

2. Nicewonger RB, Ditto L, Kerr D, Varady L. 2002. Synthesis of a novel, recyclable, solid-Phase acylating reagent. Bioorganic & Medicinal Chemistry Letters. 12(14):1799-1802. https://doi.org/10.1016/s0960-894x(02)00272-x

3. Sambrook J, Russell D. 2001. Molecular Cloning: A Laboratory Manual. 3rd edition, pp. 1.151, 6.23- 6.30, 7.4-7.12, A8.27.. CSHL Press: Cold Spring Harbor, NY.

4. ROMAO MJ, BARATA BAS, ARCHER M, LOBECK K, MOURA I, CARRONDO MA, LeGALL J, LOTTSPEICH F, HUBER R, MOURA JJG. 1993. Subunit composition, crystallization and preliminary crystallographic studies of the Desulfovibrio gigas aldehyde oxidoreductase containing molybdenum and [2Fe-2S] centers. Eur J Biochem. 215(3):729-732. https://doi.org/10.1111/j.1432-1033.1993.tb18085.x

5. Knapp S, Rüdiger A, Antranikian G, Jorgensen PL, Ladenstein R. 1995. Crystallization and preliminary crystallographic analysis of an amylopullulanase from the hyperthermophilic archaeonPyrococcus woesei. Proteins. 23(4):595-597. https://doi.org/10.1002/prot.340230416

6. Yang F, Bewley CA, Louis JM, Gustafson KR, Boyd MR, Gronenborn AM, Clore G, Wlodawer A. 1999. Crystal structure of cyanovirin-N, a potent HIV-inactivating protein, shows unexpected domain swapping. Journal of Molecular Biology. 288(3):403-412. https://doi.org/10.1006/jmbi.1999.2693

7. Schmidt CK, Brouwer A, Nau H. 2003. Chromatographic analysis of endogenous retinoids in tissues and serum. Analytical Biochemistry. 315(1):36-48. https://doi.org/10.1016/s0003-2697(02)00662-0

8. McCaffery P, Evans J, Koul O, Volpert A, Reid K, Ullman MD. 2002. Retinoid quantification by HPLC/MSn. Journal of Lipid Research. 43(7):1143-1149. https://doi.org/10.1194/jlr.d200012-jlr200

9. Du CM, Valko K, Bevan C, Reynolds D, Abraham MH. 2000. Characterizing the Selectivity of Stationary Phases and Organic Modifiers in Reversed-Phase High-Performance Liquid Chromatographic Systems by a General Solvation Equation Using Gradient Elution. Journal of Chromatographic Science. 38(11):503-511. https://doi.org/10.1093/chromsci/38.11.503