HPLC Analysis of Atorvastatin and Related Compound E on Kromasil® 5-AmyCoat Chiral
CONDITIONS
column
Kromasil 5-AmyCoat, 25 cm x 4.6 mm, 5 μm particles (K08670348)
mobile phase
84:16 hexane: ethanol with 0.1% TFA
flow rate
1 mL/min
pressure
1117 psi (77 bar)
column temp.
25 °C
detector
UV, 254 nm
injection
20 μL
sample
50 μg/mL atorva
Description
Analysis Note
Atorvastatin calcium also known as HMG-CoA reductase inhibitors in a class of lipid-lowering medications that reduces levels of “bad” cholesterol (low-density lipoprotein, or LDL) and triglycerides in the blood and raise “good” cholesterol (high-density lipoprotein, or HDL). The enantiomer of atorvastatin (related compound E) could qualitatively and quantitatively differ in their activities, in pharmacokinetic behavior or pharmacodynamics. The application exhibits baseline resolution under 9 minutes of a racemic mixture of atorvastatin calcium and related compound E on a 5.0 μm Kromasil 5-AmyCoat Chiral.
Legal Information
Kromasil is a registered trademark of EKA Chemicals AB