Skip to Content

The Role of Charge Density and Hydrophobicity on the Biocidal Properties of Self-Protonable Polymeric Materials.

The Role of Charge Density and Hydrophobicity on the Biocidal Properties of Self-Protonable Polymeric Materials.

Macromolecular bioscience (2015-03-18)

Simona Matrella, Carmela Vitiello, Massimo Mella, Giovanni Vigliotta, Lorella Izzo

ABSTRACT

Intrinsic antimicrobial thermoplastic A(BC)n copolymers (n = 1, 2, 4), where A was poly(ethylene glycol) (PEG), BC was a random chain of methylmethacrylate (MMA), and alkyl-aminoethyl methacrylate (AAEMA), were synthesized and the antimicrobial activity and hemolyticity were evaluated on plaques obtained by casting as a function of the architecture, the N-substituent groups of the AAEMAs (methyl, ethyl, isopropyl, and tert-butyl groups) and the hydrophobic/charge density balance. Antimicrobial effectiveness and efficiency is controlled by the surface charge density and by the influence of N-alkyl groups on the surface morphology. Also interestingly, it is the absence of hemolitytic activity in all copolymers. In presence of Escherichia coli, the A(BC)2 copolymer with 40% of N-methyl groups is the most efficient, killing 91% of the bacteria already after 1.5 h.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Chloroform, AR, contains 100 ppm amylene as stabilizer, ≥99.5%

Sigma-Aldrich

Acetone, LR, ≥99%

Sigma-Aldrich

Methanol, anhydrous, ≥99.5%

Sigma-Aldrich

Acetone, AR, ≥99.5%

Sigma-Aldrich

Methanol, LR, ≥99%

Sigma-Aldrich

Methanol, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Methanol, AR, ≥99.5%

Sigma-Aldrich

Diethyl ether, AR, contains 5 ppm BHT as stabilizer, ≥99.5%

Sigma-Aldrich

Diethyl ether, LR, contains 5 ppm BHT as stabilizer, ≥99.5%

Sigma-Aldrich

Triethylamine, LR, ≥99%

Sigma-Aldrich

Chloroform, LR, contains 100 ppm amylene as stabilizer, ≥99%

Sigma-Aldrich

Chloroform, AR, contains 1-2% ethanol as stabilizer, ≥99.5%

Sigma-Aldrich

Diethyl ether, anhydrous, contains 5 ppm BHT as stabilizer, ≥99.5%

Supelco

Diethyl ether, suitable for HPLC, contains 5 ppm BHT as stabilizer, ≥99%

Sigma-Aldrich

Methanol, NMR reference standard

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%, poly-coated bottles

Sigma-Aldrich

Triethylamine, purum, ≥99% (GC)

Sigma-Aldrich

Acetone, ≥99.5%, ACS reagent

Sigma-Aldrich

Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%

Sigma-Aldrich

Acetone, suitable for HPLC, ≥99.9%

Supelco

Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

Methanol, anhydrous, 99.8%

Sigma-Aldrich

Diethyl ether

Sigma-Aldrich

Benzaldehyde dimethyl acetal, ≥98%, FG

Sigma-Aldrich

Chloroform, ACS reagent, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

Hexadecyltrimethylammonium chloride, ≥98.0% (NT)

Sigma-Aldrich

Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, for amino acid analysis, ≥99.5% (GC)

Sigma-Aldrich

Triethylamine, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Acetone, ≥99%, meets FCC analytical specifications