In situ nanofabrication of hybrid PEG-dendritic-inorganic nanoparticles and preliminary evaluation of their biocompatibility.

In situ nanofabrication of hybrid PEG-dendritic-inorganic nanoparticles and preliminary evaluation of their biocompatibility.

Nanoscale (2014-12-23)

Ana Sousa-Herves, Christian Sánchez Espinel, Amir Fahmi, África González-Fernández, Eduardo Fernandez-Megia

ABSTRACT

An in situ template fabrication of inorganic nanoparticles using carboxylated PEG-dendritic block copolymers of the GATG family is described as a function of the dendritic block generation, the metal (Au, CdSe) and metal molar ratio. The biocompatibility of the generated nanoparticles analysed in terms of their aggregation in physiological media, cytotoxicity and uptake by macrophages relates to the PEG density of the surface of the hybrids.

MATERIALS

Product Number

Brand

Product Description

11140

Sigma-Aldrich

(+)-Sodium L-ascorbate, BioXtra, ≥99.0% (NT)

71631

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Sodium bicarbonate, tested according to Ph. Eur.

60089

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451010

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Potassium bromide, anhydrous, powder, 99.95% trace metals basis

449962

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A4034

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(+)-Sodium L-ascorbate, powder, BioReagent, suitable for cell culture

A7631

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(+)-Sodium L-ascorbate, crystalline, ≥98%

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PHR1279

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221864

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13433

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Sodium bicarbonate, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E500, 99.0-100.5%, powder

900506

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tert-Butanol, AR

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tert-Butanol, puriss. p.a., ACS reagent, ≥99.7% (GC)

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