- Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants.
Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants.
Applied and environmental microbiology (2011-11-15)
Michael J Tessaro, Sameh S M Soliman, Manish N Raizada
PMID22081561
ABSTRACT
A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.
MATERIALS