- Simultaneous immobilization of cytochrome P-450 and glucuronyltransferase for synthesis of drug metabolites.
Simultaneous immobilization of cytochrome P-450 and glucuronyltransferase for synthesis of drug metabolites.
Drug metabolism and disposition: the biological fate of chemicals (1981-01-01)
J P Lehman, L Ferrin, C Fenselau, G S Yost
PMID6111425
ABSTRACT
Cytochrome P-450, NADPH-cytochrome P-450 reductase, and glucuronyltransferase were immobilized simultaneously on cyanogen bromide-activated Sepharose from phenobarbital-induced rabbit liver microsomes. The activity of the P-450 system was demonstrated by the N-demethylation of ethylmorphine and the O-demethylation of p-nitroanisole. p-Nitrophenol produced from the oxidation of p-nitroanisole was conjugated to p-nitrophenyl glucuronide, as evidenced by isolation and characterization of the glucuronide by GC/MS.