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  • Oxoester oxidoreductase activities in new isolates of Pichia anomala from apple, grape and cane juices.

Oxoester oxidoreductase activities in new isolates of Pichia anomala from apple, grape and cane juices.

FEMS yeast research (2005-03-23)
Francisco Pérez-Mendoza, Francisco Ruiz-Terán, Blanca Escudero Abarca, Arturo Navarro-Ocaña, Guadalupe Aguilar-Uscanga, Gerardo Valerio-Alfaro
ABSTRACT

Thirty-nine yeasts isolated from apple, grape and cane juices were screened for their oxidoreductase activity. The two strains of Pichia, one isolated from apple and one from cane juices, appear to be promising strains for oxidoreductase activity on alpha-oxoesters. They showed similar high yields in converting ethyl pyruvate to ethyl lactate as Saccharomyces spp. (86.6% and 85.3% versus 86.6%), and higher yields in the reduction of alpha-oxocarboxylic esters (ketopantolactone to pantolactone: 74% and 73.3%, respectively) compared to Saccharomyces spp. (yield 60%).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ethyl lactate, ≥98%, FCC, FG
Sigma-Aldrich
(−)-Ethyl L-lactate, 98%
Sigma-Aldrich
Ethyl lactate, natural, ≥98%, FCC, FG
Sigma-Aldrich
(−)-Ethyl L-lactate, purum, ≥98.0% (sum of enantiomers, GC)
Sigma-Aldrich
(−)-Ethyl L-lactate, photoresist grade, ≥99.0%