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Common Markers and Small Molecule Inhibitors in Golgi Studies.

Methods in molecular biology (Clifton, N.J.) (2022-12-14)
Sarah Bui, Drew Stark, Jie Li, Jianchao Zhang, Yanzhuang Wang
ABSTRACT

In this chapter, we provide a detailed guide for the application of commonly used small molecules to study Golgi structure and function in vitro. Furthermore, we have curated a concise, validated list of endomembrane markers typically used in downstream assays to examine the consequent effect on the Golgi via microscopy and western blot after drug treatment. This chapter will be useful for researchers beginning their foray into the field of intracellular trafficking and Golgi biology.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tunicamycin, Streptomyces lysosuperficus
Roche
Antipain dihydrochloride, crystalline, suitable for cell culture, pkg of 10 mg
Sigma-Aldrich
Lactacystin, ≥90% (HPLC)
Sigma-Aldrich
KN-93, ≥98% (HPLC)
Sigma-Aldrich
Latrunculin B from Latruncula magnifica, ≥80% (HPLC), solid
Sigma-Aldrich
LY-411575, ≥97% (HPLC)
Sigma-Aldrich
Epoxomicin, ≥95% (HPLC), solid
Sigma-Aldrich
Etoposide, synthetic, 95.0-105.0%, powder
Sigma-Aldrich
ML-7, powder
Sigma-Aldrich
FIBRONECTIN FROM HUMAN PLASMA, liquid, 0.1% (Solution), BioReagent, suitable for cell culture
Sigma-Aldrich
N-Acetyl-L-cysteine, Sigma Grade, ≥99% (TLC), powder
Sigma-Aldrich
Rapamycin, ≥95% (HPLC), powder
Roche
Chymostatin