- In-vitro engineered human cerebral tissues mimic pathological circuit disturbances in 3D.
In-vitro engineered human cerebral tissues mimic pathological circuit disturbances in 3D.
Communications biology (2022-03-25)
Aref Saberi, Albert P Aldenkamp, Nicholas A Kurniawan, Carlijn V C Bouten
PMID35322168
ABSTRACT
In-vitro modeling of brain network disorders such as epilepsy remains a major challenge. A critical step is to develop an experimental approach that enables recapitulation of in-vivo-like three-dimensional functional complexity while allowing local modulation of the neuronal networks. Here, by promoting matrix-supported active cell reaggregation, we engineered multiregional cerebral tissues with intact 3D neuronal networks and functional interconnectivity characteristic of brain networks. Furthermore, using a multi-chambered tissue-culture chip, we show that our separated but interconnected cerebral tissues can mimic neuropathological signatures such as the propagation of epileptiform discharges.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Anti-OLIG2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Monoclonal Anti-VGLUT1 antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL2754, purified immunoglobulin, buffered aqueous glycerol solution