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Characterization of TolC efflux pump proteins from Pasteurella multocida.

Characterization of TolC efflux pump proteins from Pasteurella multocida.

Antimicrobial agents and chemotherapy (2008-08-30)

Tamas Hatfaludi, Keith Al-Hasani, Michelle Dunstone, John Boyce, Ben Adler

ABSTRACT

Two TolC homologs, PM0527 and PM1980, were identified for Pasteurella multocida. A pm0527 mutant displayed increased susceptibility to a range of chemicals, including rifampin (512-fold) and acridine orange (128-fold). A pm1980 mutant showed increased susceptibility to rifampin, ceftazidime, and vancomycin.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Nalidixic acid, ≥98%

Sigma-Aldrich

Deoxycholic acid, ≥98% (HPLC)

Sigma-Aldrich

Ethidium bromide, ~95% (HPLC)

Sigma-Aldrich

L-(−)-Dithiothreitol, ≥95% (titration)

Sigma-Aldrich

Crystal Violet, ACS reagent, ≥90.0% anhydrous basis

Sigma-Aldrich

Crystal Violet, certified by the Biological Stain Commission

Supelco

Crystal Violet, VETRANAL^®, analytical standard

Sigma-Aldrich

Gentian violet, for microscopy (Bact., Hist.)

Supelco

Sulfathiazole, VETRANAL^®, analytical standard

Sigma-Aldrich

Crystal Violet, for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)

Sigma-Aldrich

Ethidium bromide, BioReagent, for molecular biology, powder

Sigma-Aldrich

Ethidium bromide solution, for fluorescence, ~1% in H₂O

Sigma-Aldrich

Acridine Orange solution, 2% in H₂O

Sigma-Aldrich

Ethidium bromide solution, BioReagent, for molecular biology, 500 μg/mL in H₂O

Sigma-Aldrich

Erythromycin, potency: ≥850 μg per mg

Sigma-Aldrich

Erythromycin, meets USP testing specifications

Sigma-Aldrich

Erythromycin, BioReagent, suitable for cell culture

Supelco

Trimethoprim, VETRANAL^®, analytical standard

Supelco

Cycloheximide, PESTANAL^®, analytical standard

Sigma-Aldrich

Cycloheximide, from microbial, ≥94% (TLC)

Sigma-Aldrich

Cycloheximide, Biotechnology Performance Certified