Gel electrophoresis

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Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System

Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.

Cleaning Up Samples using 2-D Clean-Up Kit

2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.

How to Run an mPAGE™ Protein Gel Using an Invitrogen XCell Surelock® Mini-Cell Electrophoresis System

Instructions for setting up an mPAGE™ SDS-PAGE precast protein gel for gel electrophoresis using an Invitrogen XCell Surelock® mini-cell.

Paper-Bridge Loading

This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products

Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

Formamide Molecular Biology Reagent

Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.

DNA Detection

Nancy-520 for DNA Detection and Quantitation

Reproducibility with Biological Buffers

Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.

Components of Rehydration Solution

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels

This page shows how to apply equilibrated Immobiline DryStrip gels

Precipitation Procedures

This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Introduction to SDS-PAGE - Separation of Proteins Based on Size

Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.

ReadyBlue Protein Gel Staining Protocols

ReadyBlue™ Protein Gel Stain offers rapid Coomassie® staining for polyacrylamide gels with a simplified protocol.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Two-Dimensional Separation of Protein Samples

This page describes two-dimensional separation of protein samples with Cytiva products.

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Troubleshooting 2-D DIGE Results

Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.

mPAGE^®Lux Casting System for SDS-PAGE Gels

Refresh your gel casting workflow with ready-to-use SDS-PAGE gels in just 3 minutes. Discover faster, simpler, and safer gel casting methods.

Bis Tris Polyacrylamide Gel Electrophoresis Technology

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.

Auto2D^® Gel Electrophoresis Device

Auto2D® automates difficult 2D electrophoresis methods for protein localization with high reproducibility in under two hours.

Nucleic Acid Electrophoresis Protocols & Introduction

An introduction to Nucleric Acid electrophoresis and standard protocols for Agarose gel electrophoresis for DNA, RNA & polyacrylamide gel electrophoresis for DNA.

Rehydration Solutions

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

Preparing for IEF

This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.

Inserting Gels into Ettan™ DALT Electrophoresis Units

This protocol shows how to insert gels into Ettan™ DALT electrophoresis units.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

Proteomic analysis of membrane proteins

This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

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