ZRB2564

Anti-CYP11B1 Antibody, clone 1J14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): CYPXIB1, Cytochrome P-450c11 (Cytochrome P450C11), Cytochrome P450 11B1, mitochondrial, EC:1.14.15.4, S11BH, Steroid 11-beta-hydroxylase
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1J14, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 57.57 kDa; observed mol wt ~52 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_000488
• UniProt accession no.: P15538
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... CYP11B1(1584)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1J14 is a ZooMAb^® recombinant rabbit monoclonal antibody that detects CYP11B1. It targets an epitope within 11 amino acids from the N-teminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 11 amino acids from the N-teminal half of human CYP11B1.

Application

Quality Control Testing

Evaluated by Western Blotting in human adrenal tissue lysate.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected CYP11B1 in human adrenal tissue lysate.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound CYP11B1 peptide with at least one hundred thousand-fold (100,000X) higher than with non-specific control peptide.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected CYP11B1 in human adrenal gland tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CYP11B1 in human adrenal gland tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Cytochrome P450 11B1, mitochondrial (UniProt: P15538; EC:1.14.15.4; EC:1.14.15.4; also known as CYPXIB1, Cytochrome P-450c11 (Cytochrome P450C11), Steroid 11-beta-hydroxylase, CYP11B1) is encoded by the CYP11B1 (also known as S11BH) gene (Gene ID: 1584) in human. CYP11B1 is a member of the cytochrome P450 superfamily of enzymes, which are characterized by their heme-binding domain crucial for their enzymatic activity. CYP11B1 is a mitochondrial enzyme with a structure that includes a signal sequence that directs it to the mitochondria, where it is integrated into the inner mitochondrial membrane. CYP11B1 is primarily involved in the biosynthesis of corticosteroids, specifically in the conversion of 11-deoxycortisol to cortisol in the zona fasciculata of the adrenal cortex. This enzyme′s activity is essential for the production of cortisol, which plays a key role in glucose metabolism, immune function, and stress response. The expression of CYP11B1 is restricted to the adrenal cortex, reflecting its specialized role in steroidogenesis. Its expression and activity are regulated by adrenocorticotropic hormone (ACTH), which is part of the hypothalamic-pituitary-adrenal axis, responding to physiological stress. CYP11B1 is localized to the mitochondria, specifically within the inner membrane. Overactivity of CYP11B1 or mutations that increase its activity can contribute to conditions such as Cushing′s syndrome, characterized by excessive cortisol production and associated symptoms like hypertension, obesity, and glucose intolerance. Conversely, mutations that reduce CYP11B1 activity can lead to adrenal insufficiency, where cortisol production is inadequate, impacting stress response and metabolism. Additionally, genetic variations in CYP11B1 have been implicated in the modulation of blood pressure and may contribute to the development of hypertension. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable