SK-N-AS

94092302, human nerve (bone marrow metastasis), Polygonal

• UNSPSC Code: 41106514

Properties

• product name: SK-N-AS, 94092302
• biological source: human nerve (bone marrow metastasis)
• growth mode: Adherent
• karyotype: 46
• morphology: Polygonal
• products: Not specified
• receptors: Vimentin
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human neuroblastoma

Cell Line Description

Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.

Application

Neuroblastoma differentiation studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17

Culture Medium

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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