Recommended Products
product name
COLO 677, 93072109
biological source
human blood
growth mode
Suspension
karyotype
Not specified
morphology
Round & Refractile
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human multiple myeloma
Cell Line Description
COLO 677 was originally described as being derived from a tumour in the left axillary lymph node of a 39-year-old male with small lung cell carcinoma in 1989. However, DNA fingerprinting suggests cross-contamination with cell line RPMI-8226; RPMI-8226 was established from the peripheral blood of a 61-year-old male with multiple myeloma in 1966 (see DSMZ website)
Application
Tumourigenicity studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Will form clusters in suspension.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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