This product contains 0.25% trypsin in Hanks Balanced Salt Solution with phenol red and without Calcium or Magnesium. It may be diluted to 0.1% using HBSS products H6648, H2387, or similar.
T4049
Trypsin-EDTA solution
0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
Synonym(s):
Cocoonase, Tryptar, Tryptase
About This Item
Recommended Products
biological source
Porcine
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
mol wt
23.4 kDa
concentration
0.25%
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
impurities
Porcine parvovirus, none detected (9 CFR)
pH
7.0-7.6
shipped in
dry ice
storage temp.
−20°C
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General description
Application
- in detaching HT29 human colorectal cancer cells cultured in RPMI 1640 which was supplemented with 10 % fetal calf serum, during relative cell frequency determination of high concentration samples.[1]
- to trypsinize the transient transfected human embryonic kidney tcA-201 cell line.[2]
- to enzymatically release mouse fibroblasts cells (cell line L929) adhered to the scaffold, during cell culturing to assess the influence of several modified treatments of Poly(L/D)lactide 96/4 non-woven scaffolds and fibres.[3]
- to dissociate cells from the culture dish for flow cytometry analysis.[3]
Biochem/physiol Actions
Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.
Components
Caution
Preparation Note
recommended
related product
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Serum-free defined cancer stem cell media used to grow and expand cancer cells in 3D tumorsphere aggregates.
Protocols
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.
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What is the proper procedure for diluting to .1% Trypsin
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