G2N10
GenElute™ Plant Genomic DNA Miniprep Kit
sufficient for 10 purifications
Synonym(s):
Plant Genomic DNA Miniprep, Gen Elute
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About This Item
UNSPSC Code:
41105501
NACRES:
NA.55
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usage
sufficient for 10 purifications
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
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greener alternative category
storage temp.
15-25°C
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General description
The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
GenElute™ Plant Genomic DNA Miniprep Kit has been used:
- in isolation of arbuscular mycorrhizal (AM) fungal spores DNA
- in the purification of cetyl trimethyl ammonium bromide (CTAB)-extracted genomic DNA
- in genomic DNA isolation from leaves
The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
- PCR
- restriction endonuclease digestion
- cloning
- Southern blots
Features and Benefits
- Starting material: Up to 100 mg of plant tissue
- Expected yield: Up to 20 μg
- Elution volume: 100 - 200 μl
- Time required: < 40 min
- RNase treatment required: No
Principle
Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.
Other Notes
For additional information, please see www.sigma-aldrich.com/genomicdna.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Kit Components Also Available Separately
Product No.
Description
SDS
- C2112Column Preparation SolutionSDS
recommended
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
Supplementary Hazards
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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