LC/MS/MS Analysis of Tetracycline Antibiotics on Kromasil® Eternity™ C18
Materiales
Analytical column
Kromasil® Eternity™ C18 HPLC Column
2.5 μm particle size, pore size 100 Å, L × I.D. 100 mm × 2.1 mmStandard
CONDITIONS
column
Kromasil Eternity C18, 10 cm x 2.1 mm I.D., 2.5 μm particles (K08670895)
mobile phase
[A] 0.5% acetonitrile and 0.1% formic acid in water; [B] 0.1% formic acid in methanol:acetonitrile (1:1)
gradient
Held at 10% B for 0.1 min; to 20% B in 1.9 min; held at 20% B for 3.5 min; to 80% B in 0.1 min; held at 80% B for 1.0 min; to 10% B in 0.1 min; held at 10% B for 3.3 min
flow rate
0.2 mL/min
pressure
3070 psi (212 bar)
column temp.
30 °C
detector
MS/MS
injection
10 μL
sample
each compound 50 ng/mL in acetonitrile:water (11:89)
Descripción
Analysis Note
The analysis of tetracycline antibiotics can be problematic via liquid chromatography-mass spectrometry due to their chelating properties resulting in compounds′ irreversible retention. This application uses the organosilane-coated silica stationary phase that prevents unwanted binding of compounds and provides good peak shape with an LC/MS-compatible mobile phase. The method also delivers good resolution of compounds from their isobaric epi-metabolites that cannot be resolved via mass spectrometry alone.
Legal Information
Eternity is a trademark of EKA Chemicals AB
Kromasil is a registered trademark of EKA Chemicals AB