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LC/MS (TOF) Analysis of Opioids and Opiate-Dependence Management Drugs on Ascentis® Express HILIC

LC/MS (TOF) Analysis of Opioids and Opiate-Dependence Management Drugs on Ascentis® Express HILIC application for HPLC

Materiales

analytical column

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Ascentis® Express HILIC HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

Standard

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6-Acetylmorphine, 1.0 mg/mL solution

1 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

Codeine solution

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Hydromorphone solution

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

6-Acetylcodeine solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

Hydrocodone solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Morphine solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Oxycodone solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

mobile phase component

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Formato de amonio

eluent additive for LC-MS, LiChropur, ≥99.0%

CONDITIONS

column

Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 μm particles (53934-U)

mobile phase

2 mM ammonium formate in 90:10 (v:v) acetonitrile:water

flow rate

0.3 mL/min

column temp.

55 °C

detector

TOF/MS

injection

2 μL

sample

400 ng/mL in acetonitrile

Descripción

Analysis Note

The rapid analysis by HPLC with TOF-MS detection of seven opioids is shown here using an Ascentis Express HILIC column. Cerilliant CRMs were used to ensure reliable MS identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany