HPLC Analysis of Proteins on BIOshell A400 Protein C18, Hydrophobicity Comparision
Materiales
Analytical column
Referencia del producto
Descripción
Precios
standard
Referencia del producto
Descripción
Precios
Superóxido dismutasa from bovine erythrocytes
BioReagent, ≥3,000 units/mg protein, lyophilized powder, suitable for cell cultureGlucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
BioUltra, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥95% (SDS-PAGE), ≥550 units/mg protein (biuret)CONDITIONS
column
BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 μm particles (67463-U)
mobile phase
[A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75
gradient
10 to 100% B in 18 min
flow rate
0.3 mL/min
pressure
2800 psi total pressure (193 bar)
column temp.
30 °C
detector
UV, 215 nm
injection
5 μL
sample
superoxide dismutase, 0.13; a-lactalbumin, 0.13; serum albumin bovine, 0.13; ß-lactoglobulin A, 0.06; catalase, 0.13; (carbonic anhydrase, 0.13; ß-amylase, 0.13; glucose-6 phosphate dehydrogenase, 0.13 g/L in mobile phase A)
Descripción
Analysis Note
Analysis of proteins of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.
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