HPLC Analysis of Polyphenols in Nero d′Avola Red Wine on Discovery^® HS C18 (UV 280 nm)

sample/matrix

Nero d’Avola wine sample filtered prior injection through a 0.45 μm nylon membrane

column

Discovery HS C18, 15 cm x 2.1 mm I.D., 3 μm particles (569255-U)

mobile phase

[A] water, pH 3 with formic acid; [B] acetonitrile, pH 3 with formic acid

flow rate

0.2 mL/min

column temp.

30 °C

detector

UV, 280 nm

injection

5 μL