HPLC Analysis of Carotenes on Ascentis® Express RP-Amide, Methanol Mobile Phase
CONDITIONS
column
Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)
mobile phase
methanol
flow rate
1 mL/min
pressure
1653 psi (114 bar)
column temp.
30 °C
detector
UV-VIS, 454 nm
injection
5 μL
sample
V8 Splash® fruit juice was extracted with hexane, the extract dissolved 1:1 into ethanol for HPLC injection
Descripción
Analysis Note
Analysis was conducted using juice extract, detection via UV.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
V8 Splash is a registered trademark of CSC Brands, LP