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HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection)

HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection) application for HPLC

Materiales

analytical column

Referencia del producto
Descripción
Precios

53970-U

Ascentis® Express HILIC HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

standard

Referencia del producto
Descripción
Precios

C1778

Caffeine

Sigma Reference Standard, vial of 250 mg

S1626

Sorbic acid

99.0-101.0% anhydrous basis

47862

Pyridoxine Hydrochloride (B6)

analytical standard

47865-U

Nicotinamide (Niacinamide)

analytical standard

47861

Riboflavina (B2)

analytical standard

8S61336

Benzoic acid solution

2000 μg/mL in dichloromethane, analytical standard

CONDITIONS

column

Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)

flow rate

0.6 mL/min

pressure

812 psi (56 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Dilute 1:9 in acetonitrile

Descripción

Analysis Note

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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