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Merck

Volatiles in white wine

Volatiles in white wine suitable for GC, application for SPME

Materiales

Analytical column

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Columna capilar para GC VOCOL®

L × I.D. 30 m × 0.25 mm, df 1.50 μm

Related product

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Portafibra SPME

for use with manual sampling

Portafibra SPME

for use with CTC CombiPAL and Gerstel MPS2

SPME fiber

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Fibra de carboxeno/polidimetilsiloxano (DVB/CAR/PDMS) de SPME

df 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga

Standard

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Hexyl acetate

analytical standard

Ácido octanoico

analytical standard

Ethyl butyrate

analytical standard

Isoamyl alcohol

≥98%, FG

2-Metil-1-butanol

≥99%

Sulfur dioxide

≥99.9%

Ethyl hexanoate

analytical standard

Ethyl octanoate

analytical standard

2,3-Butanediol

analytical standard, mixture of racemic and meso forms

mobile phase component

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Methyl formate

analytical standard

CONDITIONS

sample/matrix

white wine + 25% NaCl

SPME fiber

Carboxen/PDMS, 75 μm (57318)

extraction

10 min, 40 °C (headspace)

desorption process

3 min at 290 °C

column

VOCOL, 30 m × 0.25 mm I.D., 1.5 μm (24205-U)

oven

40 °C (2 min) to 220 °C at 10 °C/min

inj. temp.

290 °C

detector

GC-MS, quadrupole, m/z = 31-240

carrier gas

helium, 35 cm/sec

injection

splitless/split, closed 1 min

liner

0.75 mm liner

sample

white wine

Descripción

Analysis Note

In this application, SPME was used for the analysis of volatiles in white wine using a headspace method. The presence of aroma compounds in white wine is one of the most important attributes of its final quality. A Carboxen/PDMS fiber was inserted into the headspace of the vial for 10 minutes at 40 °C and analyzed by GC/MS. The thick film of the VOCOL GC column helps focus the volatile analytes.

Legal Information

VOCOL is a registered trademark of Merck KGaA, Darmstadt, Germany