Saltar al contenido
Merck
  • Study of chemical composition and volatile compounds along with in-vitro assay of antioxidant activity of two medicinal rice varieties: Karungkuravai and Mappilai samba.

Study of chemical composition and volatile compounds along with in-vitro assay of antioxidant activity of two medicinal rice varieties: Karungkuravai and Mappilai samba.

Journal of food science and technology (2015-04-22)
K Krishnanunni, P Senthilvel, Sudha Ramaiah, Anand Anbarasu
RESUMEN

The objective of this study is to analyze the chemical composition and volatiles present in two medicinal rice varieties of "karungkuravai" and "mappilai samba" and to investigate their total phenolic content and bio activity. Chemical composition of the rice varieties was analyzed using gas chromatography/mass spectroscopy (GC-MS), the volatile compounds are identified using static head space analysis (SHS) followed by GC-MS. Total phenolic content (TPC) is estimated using Folin Ciocalteu colorimetric method, antioxidant assay is done using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay and ferric reducing antioxidant power (FRAP) assay. GC-MS and SHS analysis identified pharmaceutically important phytochemicals present in the rice variety. The compounds like "curlone" and "7-chloro-1,3-dihydro-5-phenyl-1-(trimethylsilyl)- 2H-1-4-benzodiazapene-2-one" are identified for the first time in any rice variety. Phenols are found to be present in both rice varieties. Both rice varieties also showed antioxidant activity in both DPPH and FRAP assays and the IC50 values were 91.08 μg/ml and 359.93 μg/ml for "karungkuravai" and "mappilai samba", respectively. . The correlation coefficient and regression analysis of total phenolic content with DPPH assay and FRAP assay show significant positive correlation coefficient values and coefficient of regression values.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Alcohol etílico puro, for molecular biology
Sigma-Aldrich
Ácido clorhídrico, ACS reagent, 37%
Sigma-Aldrich
Alcohol etílico puro, 200 proof, ACS reagent, ≥99.5%
Sigma-Aldrich
Alcohol etílico puro, 200 proof
Sigma-Aldrich
Ácido clorhídrico, ACS reagent, 37%
Sigma-Aldrich
Cloruro de hidrógeno solution, 4.0 M in dioxane
Sigma-Aldrich
Alcohol etílico puro, 200 proof, meets USP testing specifications
Sigma-Aldrich
2,2-difenil-1-picrilhidrazil
Sigma-Aldrich
Alcohol etílico puro, for molecular biology
Sigma-Aldrich
Ácido clorhídrico solution, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Ácido clorhídrico, meets analytical specification of Ph. Eur., BP, NF, fuming, 36.5-38%
Sigma-Aldrich
Ácido clorhídrico, 37 wt. % in H2O, 99.999% trace metals basis
Sigma-Aldrich
Ácido clorhídrico, 36.5-38.0%, BioReagent, for molecular biology
Sigma-Aldrich
Cloruro de hidrógeno solution, 2.0 M in diethyl ether
Sigma-Aldrich
Ácido clorhídrico, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., fuming, ≥37%, APHA: ≤10
Sigma-Aldrich
Etanol, ACS reagent, prima fine spirit, without additive, F15 o1
Supelco
Ácido clorhídrico solution, volumetric, 0.1 M HCl (0.1N), endotoxin free
Sigma-Aldrich
Cloruro de hierro (III), anhydrous, powder, ≥99.99% trace metals basis
Sigma-Aldrich
Cloruro de hidrógeno solution, 1.0 M in diethyl ether
Sigma-Aldrich
Cloruro de hierro (III), sublimed grade, ≥99.9% trace metals basis
Sigma-Aldrich
Ácido clorhídrico, puriss., 24.5-26.0%
Sigma-Aldrich
Hydrogen chloride, ReagentPlus®, ≥99%
Sigma-Aldrich
Iron(III) chloride solution, purum, 45% FeCl3 basis
Sigma-Aldrich
Ácido clorhídrico solution, ~6 M in H2O, for amino acid analysis
Sigma-Aldrich
Alcohol etílico puro, 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
Cloruro de hidrógeno solution, 3 M in cyclopentyl methyl ether (CPME)
Sigma-Aldrich
Etanol, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)
Sigma-Aldrich
Ácido clorhídrico solution, 32 wt. % in H2O, FCC
Sigma-Aldrich
Alcohol etílico puro, 200 proof, anhydrous, ≥99.5%
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol