A sensitive enzymatic assay for determination of cholesterol in lipid extracts.

A sensitive enzymatic assay for determination of cholesterol in lipid extracts.

Analytical biochemistry (1984-11-01)

F Omodeo Salè, S Marchesini, P H Fishman, B Berra

RESUMEN

A procedure for the determination of free and total cholesterol in lipid extracts is described. The method for free cholesterol employs cholesterol oxidase to generate H2O2 and peroxidase to catalyze the reaction of H2O2 with o-dianisidine to yield a colored product. For the determination of total cholesterol, cholesterol ester hydrolase is included.

MATERIALES

Referencia del producto

Marca

Descripción del producto

33430

Supelco

o-Dianisidine, for spectrophotometric det. of Au, NO₂^-, Ce(IV), for the detection of Au, Co, Cu, SCN^-, V, ≥97.0%