Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer-nucleotide coacervate droplets.

Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer-nucleotide coacervate droplets.

Chemical communications (Cambridge, England) (2012-10-27)

John Crosby, Tom Treadwell, Michelle Hammerton, Konstantinos Vasilakis, Matthew P Crump, David S Williams, Stephen Mann

RESUMEN

Compartmentalization of the minimal complex of actinorhodin polyketide synthase in coacervate liquid droplets produces enhanced yields of shunt polyketides under conditions of low and high ionic strength.

MATERIALES

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409022

Sigma-Aldrich

Poly(diallyldimethylammonium chloride) solution, average M_w 200,000-350,000 (medium molecular weight), 20 wt. % in H₂O

409014

Sigma-Aldrich

Poly(diallyldimethylammonium chloride) solution, 20 wt. % in H₂O

522376

Sigma-Aldrich

Poly(diallyldimethylammonium chloride) solution, average M_w <100,000 (very low molecular weight), 35 wt. % in H₂O

409030

Sigma-Aldrich

Poly(diallyldimethylammonium chloride) solution, average M_w 400,000-500,000 (high molecular weight), 20 wt. % in H₂O