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  • Effects of lignin-derived phenolic compounds on xylitol production and key enzyme activities by a xylose utilizing yeast Candida athensensis SB18.

Effects of lignin-derived phenolic compounds on xylitol production and key enzyme activities by a xylose utilizing yeast Candida athensensis SB18.

Bioresource technology (2012-08-07)
Jinming Zhang, Anli Geng, Chuanyi Yao, Yinghua Lu, Qingbiao Li
RESUMEN

Candida athensensis SB18 is potential xylitol producing yeast isolated in Singapore. It has excellent xylose tolerance and is able to produce xylitol in high titer and yield. However, by-products, such as phenolic compounds, derived in lignocellulosic biomass hydrolysate might negatively influence the performance of this strain for xylitol production. In this work, four potential phenolic inhibitors, such as vanillin, syringaldehyde, 4-hydroxybenzaldehyde and phenol, were evaluated for their inhibitory effects on xylitol production by C. athensensis SB18. Phenol was shown to be the most toxic molecule on this microorganism followed by syringaldehyde. Vanillin and 4-hydroxylbenzaldehyde was less toxic than phenol and syringaldehyde, with vanillin being the least toxic. Inhibition was insignificant when the total content of inhibitors was below 1.0 g/L. The presence of phenolic compounds affected the activity of xylose reductase, however not on that of xylitol dehydrogenase. C. athensensis SB18 is therefore a potential xylitol producer from hemicellulosic hydrolysate due to its assimilation of such phenolic inhibitors.

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Sigma-Aldrich
4-Hydroxybenzaldehyde, 98%
Sigma-Aldrich
Vainillina, ReagentPlus®, 99%
Sigma-Aldrich
Syringaldehyde, 98%
Sigma-Aldrich
Vainillina, natural, ≥97%, FCC, FG
Supelco
Vainillina, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Vainillina, ≥97%, FCC, FG
Sigma-Aldrich
4-Hydroxybenzaldehyde, ≥97%, FG
Sigma-Aldrich
Syringaldehyde, ≥98%, FG
Sigma-Aldrich
4-Hydroxybenzaldehyde, ≥95.0% (HPLC)
Supelco
Vainillina, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Vainillina, traceable to primary standards (LGC)
Sigma-Aldrich
Vainillina, tested according to Ph. Eur.
Vainillina, European Pharmacopoeia (EP) Reference Standard