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Merck

High yield sample preconcentration using a highly ion-conductive charge-selective polymer.

Analytical chemistry (2010-06-26)
Honggu Chun, Taek Dong Chung, J Michael Ramsey
RESUMEN

The development and analysis of a microfluidic sample preconcentration system using a highly ion-conductive charge-selective polymer [poly-AMPS (2-acrylamido-2-methyl-1-propanesulfonic acid)] is reported. The preconcentration is based on the phenomenon of concentration polarization which develops at the boundaries of the poly-AMPS with buffer solutions. A negatively charged polymer, poly-AMPS, positioned between two microchannels efficiently extracts cations through its large cross section, resulting in efficient anion sample preconcentration. The present work includes the development of a robust polymer that is stable over a wide range of buffers with varying chemical compositions. The sample preconcentration effect remains linear to over 3 mM (0.15 pmol) and 500 microM (15 fmol) for fluorescein and TRITC-tagged albumin solutions, respectively. The system can potentially be used for concentrating proteins on microfluidic devices with subsequent analysis for proteomic applications.

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2-Acrylamido-2-methyl-1-propanesulfonic acid, 99%
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2-Acrylamido-2-methyl-1-propanesulfonic acid sodium salt solution, 50 wt. % in H2O
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