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Merck

Aptamer-mediated chemiluminescence detection of prion protein on a membrane using trimethoxyphenylglyoxal.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2010-06-15)
Md Towhid Hossain, Takayuki Shibata, Tsutomu Kabashima, Masaaki Kai
RESUMEN

Effective recognition and quantitative analysis of the prion protein are important in drug discovery and diagnosis for prion diseases, such as bovine spongiform encephalopathy and Creutzfeldt-Jakob diseases. We have developed a high-throughput method for a specific and sensitive determination of prion protein on a solid-phase membrane, based on a chemiluminescence reaction of aptamer with 3,4,5-trimethoxyphenylglyoxal. This method using aptamer is facile, inexpensive and convenient for the detection of the prion protein on a membrane, indicating a lower detection limit of ca. 4.2 pmol spot(-1).

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Sigma-Aldrich
Phenylglyoxal monohydrate, crystallized, ≥97.0% (GC)