- Modification of the selected method for the determination of serum iron. Substitution of bathophenanthroline by ferene S.
Modification of the selected method for the determination of serum iron. Substitution of bathophenanthroline by ferene S.
Annales de biologie clinique (1992-01-01)
C Charlier, G Plomteux, M Vernet, P Gendre, M C Revenant, C Guillemin
PMID1456498
RESUMEN
The previously selected method (1977) for the measurement of iron in human serum has been modified by the working group on Iron and Iron-Transport Proteins (Sociéte Française de Biologie Clinique). Ferene S is used as the chromogen, it is more sensitive and cheaper than bathophenanthroline. The sample size has been reduced from 2 to 0.5 ml. No interference could be shown with high concentrations of bilirubin, copper or hemoglobin.
MATERIALES
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Supelco
3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt, for spectrophotometric det. of Fe, ≥99.0%
Sigma-Aldrich
3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt, Suitable for determination of Fe