Saltar al contenido
Merck
  • IL-4 Inhibits the Biogenesis of an Epigenetically Suppressive PIWI-Interacting RNA To Upregulate CD1a Molecules on Monocytes/Dendritic Cells.

IL-4 Inhibits the Biogenesis of an Epigenetically Suppressive PIWI-Interacting RNA To Upregulate CD1a Molecules on Monocytes/Dendritic Cells.

Journal of immunology (Baltimore, Md. : 1950) (2016-01-13)
Xue Zhang, Xin He, Chao Liu, Jun Liu, Qifei Hu, Ting Pan, Xiaobing Duan, Bingfeng Liu, Yiwen Zhang, Jingliang Chen, Xingru Ma, Xu Zhang, Haihua Luo, Hui Zhang
RESUMEN

The discovery of PIWI-interacting RNAs (piRNAs) revealed the complexity of the RNA world. Although piRNAs were first deemed to be germline specific, substantial evidence shows their various roles in somatic cells; however, their function in highly differentiated immune cells remains elusive. In this study, by initially screening with a small RNA deep-sequencing analysis, we found that a piRNA, tRNA-Glu-derived piRNA [td-piR(Glu)], was expressed much more abundantly in human monocytes than in dendritic cells. By regulating the polymerase III activity, IL-4 potently decreased the biogenesis of tRNA-Glu and, subsequently, td-piR(Glu). Further, we revealed that the td-piR(Glu)/PIWIL4 complex recruited SETDB1, SUV39H1, and heterochromatin protein 1β to the CD1A promoter region and facilitated H3K9 methylation. As a result, the transcription of CD1A was significantly inhibited. Collectively, we demonstrated that a piRNA acted as the signal molecule for a cytokine to regulate the expression of an important membrane protein for lipid Ag presentation.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Anti-PIWIL1 antibody, Mouse monoclonal, clone PIWIL1-2, purified from hybridoma cell culture