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ZRB2239

Sigma-Aldrich

Anti-C1QBP Antibody, clone 3D2 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinónimos:

ASF/SF2-associated protein p32, Complement component 1 Q subcomponent-binding protein, mitochondrial, Glycoprotein gC1qBP, Hyaluronan-binding protein 1, Mitochondrial matrix protein p32, SF2AP32, gC1q-R protein, p33

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

recombinant monoclonal

description

clone 3D2
recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 31.36 kDa
observed mol wt ~31 kDa

purified by

using Protein A

species reactivity

mouse, human

packaging

antibody small pack of 25

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

epitope sequence

Internal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... C1QBP(708)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3D2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects C1QBP. It targets an epitope within 16 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the internal region of human C1QBP.

Application

Quality Control Testing

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected C1QBP in RAW264.7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected C1QBP in lysates from HT-1080 cells and Human testis tissue..

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected C1QBP in Human testis tissue sections.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected C1QBP in one million HT-1080 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected C1QBP in HT1080 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Complement component 1 Q subcomponent-binding protein, mitochondrial (UniProt: Q07021; also known as ASF/SF2-associated protein p32, Glycoprotein gC1qBP, C1qBP, Hyaluronan-binding protein 1, Mitochondrial matrix protein p32, gC1q-R protein, p33, SF2AP32) is encoded by the C1QBP (also known as GC1QBP, HABP1, SF2P32) gene (Gene ID: 708) in human. C1QBP is a highly conserved, multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, mitochondrial protein synthesis, regulation of apoptosis, and transcriptional regulation and pre-mRNA splicing. Its high expression is observed cardiac and skeletal muscle. It is synthesized with a transit peptide (aa 1-73), which is proteolytically processed after it reaches the mitochondrial matrix. It is predominantly localized to mitochondria, but is also detected in cytoplasm, membrane, and nucleus. It primarily localized in mitochondrial matrix and is associated with mitochondrial oxidative phosphorylation. It also supports translation of the mitochondrially encoded respiratory chain protein complexes I, III, IV and V. It is also secreted by activated lymphocytes. At the cell surface is thought to act as an endothelial receptor for plasma proteins of the complement and kallikrein-kinin cascades. It is also reported to be a putative receptor for C1q; specifically binds to the globular ′heads′ of C1q thus inhibiting C1. It can also bind other plasma proteins, such as coagulation factor XII leading to its autoactivation. The secreted form may enhance both extrinsic and intrinsic coagulation pathways. Mutations in C1QBP gene has been linked to combined oxidative phosphorylation deficiency 33 (COXPD33), an autosomal recessive disorder leading to multiple mitochondrial respiratory chain defects and impaired mitochondrial energy metabolism. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, J., et al. (2022). Front. Cardiovasc. Med, 9; 843853; René G Feichtinger, RG., et al. (2017). Am. J. Hum. Genet. 101(4); 525-538).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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