XNAB
REDExtract-N-Amp™ Blood PCR Kit
sufficient for 100 extractions, sufficient for 100 amplifications
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
41106303
NACRES:
NA.55
Productos recomendados
usage
sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions
feature
dNTPs included
hotstart
technique(s)
PCR: suitable
color
red
shipped in
wet ice
storage temp.
−20°C
¿Está buscando productos similares? Visita Guía de comparación de productos
General description
The REDExtract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR (polymerase chain reaction). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
Application
REDExtract-N-Amp™ Blood PCR Kit has been used to extract genomic DNA from blood samples, peripheral blood leukocytes, endocervical swab eluates of mice and parasites. It has also been used to perform polymerase chain reaction (PCR) using oligonucleotide primers.
Features and Benefits
- Efficient 8 minute prep allows greater speed and throughput
- No need for organic extraction, centrifugation or alcohol precipitation
- Hot start antibody for highly specific PCR amplification of genomic DNA
- Can be used with whole blood or blood cards
- Extract stable at 4 °C for at least 6 months
- Compatible with any format (single tube, 96-well, etc.)
- Simple, 3 step procedure with no special equipment required
- REDExtract-N-Amp™ requires no loading buffers or tracking dyes required for gel analysis
- PCR products are suitable for direct cloning and sequencing
Principle
Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.
Other Notes
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Legal Information
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Related product
Referencia del producto
Descripción
Precios
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Olivier A E Sparagano et al.
Annals of the New York Academy of Sciences, 1081, 505-508 (2006-12-01)
Our study is based on the collection of blood and ticks from sheep in Iran and Italy. Polymerase chain reaction (PCR) testing was performed to target the 18S rRNA gene and RLB was performed using previously published probes. In Italy
Lung-Cheng Lin et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 17(6), 1460-1469 (2008-06-19)
The urinary benzene metabolites, trans, trans-muconic acid (ttMA) and S-phenylmercapturic acid (SPMA), are widely used as benzene exposure biomarkers. The influence of the glutathione S-transferase (GST) genetic polymorphism on the excretion levels of urinary ttMA and/or SPMA has been investigated.
Anna K Nowińska et al.
Molecular vision, 17, 2333-2342 (2011-09-17)
To analyze genotype-phenotype correlation in patients originating from Polish population with the transforming growth factor beta induced (TGFBI) corneal dystrophies. Sixty affected and 31 unaffected individuals from 15 unrelated Polish families were included in the study. The clinical diagnosis was
Dawn M Reeves et al.
Antimicrobial agents and chemotherapy, 51(7), 2646-2648 (2007-05-16)
Mice treated with antibiotics early or late after active infection had resolved were examined for chlamydial DNA in endocervical swabs. The early eradication of infection limited oviduct pathology, despite the continued detection of chlamydial DNA by nested PCR. Late antibiotic
Kosei Moriyama et al.
Microbiology and immunology, 52(7), 375-382 (2008-08-01)
Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35-40
Artículos
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico