Este artículo	FLAGIPT1	F2426	A2220
Millipore TP0010 FLAG^® HA Tandem Affinity Purification Kit	Millipore FLAGIPT1 FLAG^® Immunoprecipitation Kit	Millipore F2426 EZview^™ Red ANTI-FLAG^® M2 Affinity Gel	Millipore A2220 ANTI-FLAG^® M2 Affinity Gel
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200

General description

TAP technology is a recent development in protein purification. This technology incorporates tandem-linked affinity tags into genes of interest so that high purity fusion proteins can be isolated through two consecutive affinity purification steps. The technology is particularly useful for the isolation and identification of protein complexes by adding a tandem affinity tag to a known "bait" protein to pull down endogenous proteins that interact with the targeted protein of interest.

Application

The FLAG^® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology.

Sutitable for mass spectrometry analysis and western blotting.

Learn more product details in our FLAG^® application portal.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Los componentes del kit también están disponibles por separado

Referencia del producto

Descripción

SDS

F2426EZview^™ Red ANTI-FLAG^® M2 Affinity Gel, clone M2SDS

A2095Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspensionSDS

R0278RIPA BufferSDS

F47993X FLAG^® Peptide, lyophilized powderSDS

U4883Urea, 8 M (after reconstitution with 16 mL high purity water)SDS

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

pictograms

GHS07

signalword

Danger

hcodes

H319,H412,EUH430

pcodes

P201 - P202 - P273 - P391 - P405 - P501

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2

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Merlin/NF2 suppresses tumorigenesis by inhibiting the E3 ubiquitin ligase CRL4(DCAF1) in the nucleus.

Wei Li et al.

Cell, 140(4), 477-490 (2010-02-25)

Current models imply that the FERM domain protein Merlin, encoded by the tumor suppressor NF2, inhibits mitogenic signaling at or near the plasma membrane. Here, we show that the closed, growth-inhibitory form of Merlin accumulates in the nucleus, binds to

Host Protein Moloney Leukemia Virus 10 (MOV10) Acts as a Restriction Factor of Influenza A Virus by Inhibiting the Nuclear Import of the Viral Nucleoprotein.

Junsong Zhang et al.

Journal of virology, 90(8), 3966-3980 (2016-02-05)

The viral ribonucleoprotein (vRNP) complex of influenza A viruses (IAVs) contains an RNA-dependent RNA polymerase complex (RdRp) and nucleoprotein (NP) and is the functional unit for viral RNA transcription and replication. The vRNP complex is an important determinant of virus

SARS-CoV-2 infection enhances mitochondrial PTP complex activity to perturb cardiac energetics.

Karthik Ramachandran et al.

iScience, 25(1), 103722-103722 (2022-01-11)

SARS-CoV-2 is a newly identified coronavirus that causes the respiratory disease called coronavirus disease 2019 (COVID-19). With an urgent need for therapeutics, we lack a full understanding of the molecular basis of SARS-CoV-2-induced cellular damage and disease progression. Here, we

Partial proteasomal degradation of Lola triggers the male-to-female switch of a dimorphic courtship circuit.

Kosei Sato et al.

Nature communications, 10(1), 166-166 (2019-01-13)

In Drosophila, some neurons develop sex-specific neurites that contribute to dimorphic circuits for sex-specific behavior. As opposed to the idea that the sexual dichotomy in transcriptional profiles produced by a sex-specific factor underlies such sex differences, we discovered that the

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