COLO 839

93051119, human blood, Lymphoblastoid

• UNSPSC Code: 41106514

Propiedades

• product name: COLO 839, 93051119
• biological source: human blood
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian B lymphocyte

Cell Line Description

Derived from the peripheral blood of a 43 year old Caucasian male with widespread melanoma. The cells were transformed with defective EBV (B-95-W8).
COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 9.3,10
TPOX: 8,11
vWA: 17,19

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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