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Merck

C8974

Sigma-Aldrich

Anti-CD27 antibody produced in goat

affinity isolated antibody, lyophilized powder

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human

technique(s)

flow cytometry: suitable
neutralization: 2.25-9.0 μg/mL
western blot: 0.1-0.2 μg/mL

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CD27(939)

General description

CD27, a member of tumour necrosis factor receptor superfamily is mainly responsible for regulation of cell proliferation and cell death. CD27 and its ligand CD70 expresses mainly on lymphocytes. Anti-CD27 antibody can be used in immunoblotting and flow cytometry. It can also be used in immunopresipitating CD27. Anti-CD27 antibody reacts specifically with human CD27 and not with recombinant human CD30, recombinant human NGF R, recombinant human soluble TNF RI, or recombinant human soluble TNF RII.

Immunogen

recombinant human CD27 extracellular domain expressed in Sf2I insect cells.

Application

Anti-CD27 antibody can be used in neutralization, immunoblotting and indirect flow cytometry assays.

Physical form

Lyophilized from 0.2 μm filtered solution of phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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K V Prasad et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(12), 6346-6351 (1997-06-10)
Members of the tumor necrosis factor receptor (TNFR) superfamily are important for cell growth and survival. In addition to providing costimulatory signals for cell proliferation, ligation of both TNFR1 and Fas can result in programmed cell death or apoptosis. The

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