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Merck

IPFL07810

Immobilon®-FL PVDF Membrane

1 roll, 7 x 8.4cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Sinónimos:

Western blotting membrane, blotting membrane, transfer membrane

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UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

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Nombre del producto

Membrana de transferencia Immobilon®- FL de PVDF, 0.45 μm pore size, 7 X 8.4 cm sheets

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

Quality Level

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General description

La membrana de transferencia Immobilon®-FL es una membrana microporosa de fluoruro de polivinilideno(PVDF) para la adsorción de proteínas transferidas desde una variedad de matrices de gel. La membrana Immobilon®-FL tiene un tamaño nominal de poro de 0,45 μm y se recomienda para la transferencia de proteínas con pesos moleculares superiores a 20 kilodalton (kDa). La membrana exhibe una autofluorescencia muy baja en una amplia gama de longitudes de onda de excitación/emisión. Esta propiedad la hace ideal para aplicaciones de transferencia que implican inmunodetección por fluorescencia.
Código del filtro: IPFL

Application

La membrana de transferencia Immobilon®-FL se ha utilizado en inmunoelectrotransferencia.[1][2][3]

Features and Benefits

Membrana de transferencia Immobilon®-FL:
  • Diseñada para la aplicación óptima de la inmunodetección fluorescente
  • El fondo extremadamente bajo mejora la sensibilidad de toda la inmunodetección basada en fluorescencia
  • Compatible con todas las sondas fluorescentes de uso común en una amplia gama de longitudes de onda de excitación/emisión
  • Para la detección quimioluminiscente o cromógena estándar

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Questions

  1. Could you confirm if the IPFL07810 Immobilon membrane is compatible with a specific imaging system? I need to verify if this product will work for my requirements.

    1 answer
    1. Yes, low-fluorescent PVDF membrane is required in Licor IR Imaging Systems. The Immobilon-FL PVDF Membrane is suitable for this purpose. Immobilon-FL Membrane is the first transfer membrane optimized for fluorescence applications, with extremely low background that improves the sensitivity of all fluorescence detection protocols. It is compatible with all commonly used fluorescent probes at all excitation and emission wavelengths, making it ideal for multiplexing and chemifluorescence applications.

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