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Microbial Cell Culture

Bacterial cell culture on agar plates

Microbial cell cultures are used in molecular biology for cloning and recombinant protein expression. They are also used in clinical applications to isolate, detect, and identify microbes that cause disease. Microbial culturing enables cell growth and division under controlled laboratory conditions. Bacteria and other microbes can be grown in liquid broth or solid nutrient agar culture media using aseptic techniques to prevent contamination.  

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Antibiotics

We offer a comprehensive portfolio of antibiotics to prevent contamination of cell cultures through pathogens or other cell lines, for genetic marker selection and cell biology research.

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Milli-Q® IQ 7003/05/10/15 Ultrapure and Pure Water Purification System - Type 1 and Type 2 lab water purification system with production and tank units plus pure and ultrapure water dispensers.
Milli-Q® Benchtop Lab Water Purification Systems

Milli-Q® systems offer innovative water purification technologies engineered to...

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A circular diagram representing a plasmid map with various labeled elements. At the top, there’s a segment labeled “CMV promoter” in dark blue, followed by an orange segment labeled “hGh polyA.” To the right, there’s a yellow segment labeled “SV40 origin.” Below that, on the right side of the circle, is a green segment labeled “amp^r,” indicating ampicillin resistance. On the bottom left is another antibiotic resistance label, “amp^k,” in red. There are two more segments: one labeled “f1 origin” in light blue on the left and another labeled “pBR322 origin” at the bottom center in purple. The title above reads ‘MCS Regions Shown Below,’ indicating that these are multiple cloning sites within the plasmid used for genetic engineering purposes.
Molecular Cloning & Protein Expression

Molecular cloning and protein expression methods and protocols for reliable cloning...

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Microbial Culture Media Raw Materials

Explore microbiological culture media raw materials and supplements, ensuring safety...

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Cloning and Recombinant Protein Expression

Bacterial cultures are used for recombinant protein expression, plasmid cloning and amplification, and bacterial artificial chromosome (BAC) and cosmid cloning. Bacterial cells can be altered to uptake and incorporate exogenous genes and plasmids through transformation. Transformation of competent cells typically occurs through electroporation or heat shock of chemically-treated competent cells.

Transformed bacteria are then identified and selected based on acquired antibiotic resistance imparted by recombinant plasmid DNA or using “blue-white” screening to identify recombinant bacteria expressing β-galactosidase as a marker. Yeast can also be transformed using electroporation and other methods for yeast two-hybrid and reporter gene assays to study protein expression and protein interactions.

Cultivation for Microbial Isolation and Identification

Microbial cultures are medically important for the isolation, detection, and differentiation of bacteria and other microbes that cause infectious disease. Characterization and identification using cultivation methods rely on both microbial phenotype and genotype. Cells can be isolated through inoculation of a streak plate to produce pure colonies. Selective media are used to inhibit the growth of certain groups of microbes, allowing others to grow. Differential media contain compounds that enable microbes to be distinguished visually by colony appearance or changes in surrounding media resulting from differences in metabolic or hemolytic activity. These media aid in the identification of bacteria and other microbes through differentiation and elimination.

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      Featured Articles
      Introduction to Microbial Media
      An introduction to microbial media types, uses and applications. Includes formulations and product selection guide.
      Antifoams
      Antifoams are supplied as two basic types of composition or mixtures of the two. Organic antifoams are of synthetic origin. Silicone-based antifoams are generally considered to be siloxane polymers and are also synthetic.
      Streptococci - Overview of Detection, Identification, Differentiation and Cultivation Techniques
      Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques
      Virus Cultivation
      Cell lines for vaccine production and cultivation of SARS CoV-2 and other viruses. Primary, human diploid, and continuous culture cell lines for the propagation of viruses and virus-based vaccines.
      Plasmid DNA | Plasmid Purification Kits
      Simplify plasmid DNA purification with kits in multiple sizes. Ideal for cloning and protein expression needs.
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