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Phyllosilicate Clay Minerals: Principles and Applications

We have discussed shortly the applications of 1:1 and 2:1 phyllosilicate material in membrane technology, as well as the basic geometric understanding of their layered structures.

Supel™-Select Polymeric SPE

Supel-Select HLB SPE - a hydrophilic modified styrene based polymer developed for the solid phase extraction of a broad range of compounds from aqueous samples.

Ascentis® Express C8 U/HPLC Columns

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.

Determination of L- and D-Lactic Acid Enantiomers by HPLC-MS-MS

HPLC Analysis of Flavonoids on Ascentis® RP-Amide

Coumaric acid; Quercitrin; Myricetin; Quercetin

Improving HILIC Mode Chromatography by Choosing the Proper Injection and Wash Solvent

Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum

Novel sample prep technique coupled with the unique selectivity of the Ascentis Express F5 column enables a fast and simplified bioanalytical method for associated vitamin D metabolites.

Evolving View of Natural Flavors

All natural is a common claim on food in the US and other regions, but what does this really mean?

Asymmetric Transfer Hydrogenation

The use of transfer hydrogenation to reduce alkenes, carboxyl groups, ketones, or imines has become very popular.

ChiPros Chiral Alcohols

Chiral alcohols form a versatile class of chiral synthons, since they can be incorporated into the API structures directly as esters or ethers.

Lithium Aminoborohydride (LAB) Reagents

Lithium aminoborohydride (LAB) reagents are a new class of powerful and selective reagents developed in the laboratory of Professor Bakthan Singaram at the University of California, Santa Cruz.

Glycol Ethers Properties & Examples, Organic Solvents

Glycol ethers, with both an ether and alcohol functional group in the same molecule, are one of the most versatile classes of organic solvents.

Troubleshooting PCR and RT-PCR Amplification

This page shows PCR and RT-PCR amplification troubleshooting.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting

The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.

ThermaGenix PCR Additives Reduce Background and Increase Yield

How to stop nonspecific bands and off target primers in PCR.

Lentivirus Cell Line

Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.

Tips and Tricks: Millicell^® DCI Digital Cell Imager Cloud Application

Tips and tricks for using the Millicell® Cloud Application to analyze data from the Millicell® DCI digital cell imager.

General Adherent Cells

Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.

Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.

Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit

Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.

Universal ProbeLibrary System Technology

UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.

DIG Labeling Methods

ChIC and CUT&RUN Data Analysis Tool Overview

Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool for file management and downstream sequencing data analyses.

DNA and RNA Quantification

An overview of methods for quantifying DNA and RNA, how to measure DNA and RNA concentration and yield, and how to assess purity of nucleic acid samples.

Oligonucleotide Quality Control by Analytical HPLC

Kapa Biosystems qPCR Reagents and Kits

Quantitative PCR (qPCR) and qRT-PCR reagents and kits that are designed to deliver maximum efficiency and reliable results from a variety of genetic materials.

A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®

Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.

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