Skip to Content
Merck

HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection)

HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53916-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 3 mm

standard

Product No.
Description
Pricing

C1778

Caffeine

Sigma Reference Standard, vial of 250 mg

S1626

Sorbic acid

99.0-101.0% anhydrous basis

47862

Pyridoxine Hydrochloride (B6)

analytical standard

47865-U

Nicotinamide (Niacinamide)

analytical standard

47861

Riboflavin (B2)

analytical standard

8S61336

SS Benzoic Acid

2000 μg/mL in dichloromethane, analytical standard

CONDITIONS

column

Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 μm particles (53916-U)

mobile phase

[A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA

flow rate

0.6 mL/min

pressure

1146 psi (79 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Energy Drink Diluted 1:9 in acetonitrile

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in caffeinated energy drinks.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS