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Surface charge switching nanoparticles for magnetic resonance imaging.

Surface charge switching nanoparticles for magnetic resonance imaging.

International journal of pharmaceutics (2014-05-27)

Dong Jin Lee, Young Taik Oh, Eun Seong Lee

ABSTRACT

In this study, polypeptide-based nanoparticles [constituted using poly(L-lysine) coupled with deoxycholic acid (DOCA) and conjugated with 2,3-dimethylmaleic acid (DMA)] have high tumor selectivity once electrostatically switched by the acidic milieu of solid tumors. These nanoparticles exhibited a significantly increased in vitro cellular uptake and high accumulation in the acidic tumor site in vivo. Consequently, Fe3O4-loaded nanoparticles enabled high contrast magnetic resonance (MR) imaging of the tumor in vivo.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Hexane, HPLC Plus, for HPLC, GC, and residue analysis, ≥95%

Millipore

Bifido Selective Supplement B, suitable for microbiology

Sigma-Aldrich

Dimethyl sulfoxide solution, 50 wt. % in H₂O

Supelco

5α-Androstan-17β-ol-3-one, VETRANAL^®, analytical standard

Sigma-Aldrich

Succinic anhydride, ≥99% (GC)

Supelco

Pyridine, analytical standard

Sigma-Aldrich

Pyridine, anhydrous, 99.8%

Sigma-Aldrich

N-Hydroxysuccinimide, 98%

Sigma-Aldrich

Hexylamine, 99%

Sigma-Aldrich

Dimethyl sulfoxide, anhydrous, ≥99.9%

Supelco

Hexane, analytical standard

Supelco

Dodecanoic acid, analytical standard

Sigma-Aldrich

Dibenzyl ether, purum, ≥98.0% (GC)

Supelco

Trifluoroacetic acid, analytical standard

Sigma-Aldrich

Hexane, puriss. p.a., ACS reagent, ≥99.0% (GC)

Sigma-Aldrich

Benzyl ether, 98%

Sigma-Aldrich

Sodium tetraborate, 99.998% trace metals basis

Sigma-Aldrich

Dodecanoic acid, 98%

Sigma-Aldrich

Sodium tetraborate, 99%

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8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)

Sigma-Aldrich

Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)

Sigma-Aldrich

Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

Sigma-Aldrich

Hexane, anhydrous, 95%

Sigma-Aldrich

Triphosgene, reagent grade, 98%

Sigma-Aldrich

Benzyl ether, ≥98%, FCC, FG

Sigma-Aldrich

N-Hydroxysuccinimide, purum, ≥97.0% (T)

Sigma-Aldrich

Pyridine, ≥99%

Supelco

Dimethyl sulfoxide, analytical standard

Sigma-Aldrich

Dodecanoic acid, ≥99% (GC/titration)

Sigma-Aldrich

Deoxycholic acid, ≥98% (HPLC)