Skip to Content
Merck
  • Nano-transfersomal ciprofloxacin loaded vesicles for non-invasive trans-tympanic ototopical delivery: in-vitro optimization, ex-vivo permeation studies, and in-vivo assessment.

Nano-transfersomal ciprofloxacin loaded vesicles for non-invasive trans-tympanic ototopical delivery: in-vitro optimization, ex-vivo permeation studies, and in-vivo assessment.

International journal of pharmaceutics (2014-06-28)
Abdulaziz Mohsen Al-Mahallawi, Omneya Mohammed Khowessah, Raguia Ali Shoukri
ABSTRACT

Ciprofloxacin is a synthetic fluoroquinolone antibiotic that has been used for systemic treatment of otitis media in adults. It was approved for topical treatment of otorrhea in children with tympanostomy tubes. The aim of this work was to enhance the local non-invasive delivery of ciprofloxacin to the middle ear across an intact tympanic membrane (TM) in an attempt to treat acute otitis media (AOM) ototopically. In order to achieve this goal, ciprofloxacin nano-transfersomal vesicles were prepared by thin film hydration (TFH) technique, using several edge activators (EAs) of varying hydrophilic-lipophilic balance (HLB) values. A full factorial design was employed for the optimization of formulation variables using Design-Expert(®) software. The optimal formulation was subjected to stability testing, ex-vivo permeation studies (through ear skin and TM of rabbits), and in-vivo evaluation. Results revealed that the optimal formulation (composed of phospholipid and sodium cholate as an EA at a molar ratio of 5:1) exhibited enhanced ex-vivo drug flux through ear skin and TM when compared with the commercial product (Ciprocin(®) drops). It demonstrated a greater extent of in-vivo drug deposition in the TM of albino rabbits relative to Ciprocin(®). Consequently, transfersomes could be promising for the non-invasive trans-tympanic delivery of ciprofloxacin.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
SyntheChol® NS0 Supplement, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Supelco
Cholesterol solution, certified reference material, 10 mg/mL in chloroform
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Sigma-Aldrich
Dihexadecyl phosphate
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Sigma-Aldrich
Cholesterol, Sigma Grade, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Sigma-Aldrich
Cholesterol, powder, BioReagent, suitable for cell culture, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Sigma-Aldrich
Cholesterol, from sheep wool, ≥92.5% (GC), powder
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Sigma-Aldrich
Octadecylamine, technical grade, 90%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Sigma-Aldrich
Octadecylamine, ≥99.0% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Sigma-Aldrich
Octadecylamine, ≥99% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
SAFC
Cholesterol, from sheep wool, Controlled origin, meets USP/NF testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Supelco
Cholesterol, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
SAFC
Cholesterol, Plant-Derived, SyntheChol®
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Sigma-Aldrich
Sorbitan monostearate, meets FCC analytical specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Sigma-Aldrich
Sodium deoxycholate, ≥97% (titration)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
Sigma-Aldrich
Sodium deoxycholate, BioXtra, ≥98.0% (dry matter, NT)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00
SAFC
Sodium deoxycholate
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
25 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$219.00
100 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$626.00
200 μL
1 Estimated to ship on March 09, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB374
$787.00