- Pseudouridine detection improvement by derivatization with methyl vinyl sulfone and capillary HPLC-mass spectrometry.
Pseudouridine detection improvement by derivatization with methyl vinyl sulfone and capillary HPLC-mass spectrometry.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2005-07-21)
Gert Emmerechts, Piet Herdewijn, Jef Rozenski
PMID16029966
ABSTRACT
A method is presented for improved detection of pseudouridine in nucleoside mixtures based on the specific derivatization with methyl vinyl sulfone followed by analysis by capillary HPLC-mass spectrometry. Reaction conditions were optimized in order to obtain the best yield and specificity. The method was successfully applied to different nucleoside mixtures.
MATERIALS