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  • Transmembrane potential measurement with carbocyanine dye diS-C3-(5): fast fluorescence decay studies.

Transmembrane potential measurement with carbocyanine dye diS-C3-(5): fast fluorescence decay studies.

Journal of photochemistry and photobiology. B, Biology (1990-01-01)
M Síp, P Herman, J Plásek, V Hrouda
ABSTRACT

The mechanism of carbocyanine dye diS-C3-(5) fluorescence intensity variations with transmembrane potential changes has been studied using time-resolved fluorescence spectroscopy. Clear evidence is given of the transmembrane-potential-dependent partition of the dye among various sites with different fluorescence lifetimes. It was found that fluorescence decay profiles reflect the transmembrane potential changes.

MATERIALS
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Product Description

Sigma-Aldrich
3,3′-Dipropylthiadicarbocyanine iodide, ≥98.0% (TLC), suitable for fluorescence