Discrimination of redox-responsible biomolecules by a single molecular sensor.

Discrimination of redox-responsible biomolecules by a single molecular sensor.

Organic letters (2013-03-07)

Jinrok Oh, Jong-In Hong

ABSTRACT

A new application of a fluorescent sensor (PyDPA) for the discrimination of redox-responsible molecules is reported. Nicotinamide adenine dinucleotide/nicotinamide adenine dinucleotide phosphate (NAD(+)/NADP(+)) and flavin mononucleotide/flavin adenine dinucleotide (FMN/FAD) were differentiated by means of ratiometric fluorescence change from excimer-monomer equilibrium and time-dependent fluorescence change, respectively.

MATERIALS

Product Number

Brand

Product Description

R0630000

Riboflavin sodium phosphate, European Pharmacopoeia (EP) Reference Standard

77623

Sigma-Aldrich

Riboflavin Sodium Phosphate Hydrate, tested according to Ph. Eur.

F1392

Sigma-Aldrich

Riboflavin 5′-monophosphate sodium salt hydrate, suitable for electrophoresis, suitable for acrylamide photopolymerization, ≥70%, powder

F6750

Sigma-Aldrich

Riboflavin 5′-monophosphate sodium salt hydrate, 73-79% (fluorimetric)

F2253

Sigma-Aldrich

Riboflavin 5′-monophosphate sodium salt hydrate, synthetic, ≥70% (HPLC)